Teagasc Bee Diagnosis Service

Teagasc offers a Honeybee disease diagnostic service to beekeepers. The following notes provide some information of the diseases tested for, tests carried out and recommendation on the proper method of collecting and forwarding samples to Teagasc for diagnosis

The bee diseases of importance in this country are Acarine, Nosema, American Foul Brood (AFB) and the presence of the Varroa mite (varroa destructor).

Diagnosis for Acarine is by examination of the first thoracic trachea. Healthy trachea are creamy white in colour. Where infestation occurs the trachea becomes discoloured ranging from slight bronzing to dark spots, circles, streaks eventually becoming black.

In the case of Nosema a sample of bees is crushed in a pestle and mortar with a small amount of water. Place a drop of the resultant liquid on a glass slide and add a cover slip. When viewed under the compound microscope at X400 magnification, Nosema spores look like grains of rice. There can be a large variation in the number of spores from one sample to another. So how many spores must be present before treatment is needed? Unfortunately there is no scientific way of resolving this problem as far as we know.

It is essential that all beekeepers can identify Varroa Destructor. Using a X10 magnification hand lens they can be seen in the floor debris, tucked in the abdominal folds of adult bees and in capped cells. If you are using open mesh floors place a white tray or piece of cardboard on the ground (beneath the floor) and check the result.

The mite also vectors virus infections and introduces transmission patterns that were not there before. Deformed Wing Viruses, Acute Bee paralysis and Slow Paralysis are the most important types.

American Foul Brood (AFB)
If you suspect AFB in a colony the following are some signs to look for before you send off the sample:

  • AFB affects only sealed brood
  • Cappings become sunken and perforated. Adult bees nibble holes in the cappings. These holes have a jagged, irregular shape.
  • If a matchstick is inserted into one of the above cells you may find a brown mucus-like thread or 'rope'.
  • The brood pattern is patchy
  • There may be a distinctive unpleasant sour smell in the latter stages of the disease
  • Larval remains dry out forming a hard black scale. These dry remains adhere down the side of the cell wall to the base.

There are two approaches to diagnosing AFB in the laboratory:

  1. Use the Vita AFB diagnostic kit. The kit is designed to provide a rapid on-site diagnosis of the disease in honeybee larvae. Clear instructions are provided with the kit.
  2. Smears of ropy material or scale are stained with a suitable bacterial stain directly onto a microscope slide. The slide is allowed to dry before microscopic examination under an oil immersion objective. Spores of AFB are often described as being similar to Nosema - i.e. rice grained in shape but considerably smaller.

The correct diagnosis of AFB depends on the sample submitted. The comb should contain as much dead or discoloured brood as possible and display some of the symptoms already described. The sample of comb should be at least 10cm by 10cm loosely wrapped in paper with cardboard exterior.

Where AFB is diagnosed, the Department of Agriculture and Food in Maynooth is notified immediately.

Collecting a sample and forwarding to Teagasc

1. Samples for bee disease diagnosis should be sent to:

Bee Disease Diagnostic Service
Dr Mary F Coffey
Teagasc Oak Park Research Centre

2. Include in clear lettering your name and address and, if you wish, your telephone number. Relevant information about the sample can be helpful: e.g. crawling/clustering bees in front of the hive. Yellow faecal spots on the frames, deformed and unhooked wings and slow build up of bee populations in Spring. Look for signs of American Foul Brood as described above. A sample form is available for printing here as a Word document - 113KB or in PDF Format - 87KB

3. If you do not know how to take a sample of your bees the officers of your local association will be glad to help

  • Send at least 28 bees from each hive
  • Take samples with a matchbox. No envelopes, plastic bags or sealed sticky containers. Squashed bees, old bees or rotten bees make dissection, particularly for Acarine disease impossible. Use a second matchbox if you have difficulty capturing 28 bees at the first attempt
  • The sample should be collected from the alighting board or from outer frames in the brood box.
  • Do not send honey to accompany the bees on their journey to the next life.
  • The bees should be killed by placing them in the fridge or for an hour in the deep freeze.
  • Even if your bees appear healthy samples should be forwarded twice a year for examination.

4. Paying
The service is free

In summary, all beekeepers should send samples of their bees at least twice each year for disease diagnosis. We should all be able to identify Varroa destructor and treat accordingly. A mighty effort should be made to rid ourselves of American Foul Brood. Examine your bees regularly and look for signs of AFB.



Co. Waterford beginners course

Co. Waterford BKA will be holding their classes for beginners starting Tuesday 21st February at Coláiste Cathal Naofa. For more information and outline of course, click here

South Tipp Beginners' Course

South Tipp BKA are holding a beginners course in beekeeping starting Saturday 8th April. For more for information on registration and what will be covered, click here

Trees for bees

**ALL TREES ARE NOW ALLOCATED** Those of you who ordered tree saplings should have already received a confirmation email by now, if not, contact Eamon at his email address

Well Done Ryan!

A Junior member of the Lake County BKA, Ryan Nea, has made it to the All Ireland Science competition and came 2nd in his college with a device which alerts beekeepers when a hive is about to swarm.......Story and picture of the young chap is the LCBKA facebook page

Gorey BKA Beginners Course

Gorey BKA Beginners Course, run by Ben Harden NBD, starts on Tuesday 4th April and continues for 4 more Tuesdays, followed by practicals in the association apiary. Venue: The Teagasc Centre, Fort Road, Gorey. Time: 8pm. Duration: approx 1hr. Bee suits are available for the practicals if required. For further details contact the secretary Aine Roche at secretary.goreybeekeepers@gmail.com

Connemara BKA Beginners Course

The Connemara Beekeepers Association are holding their beginners course in beekeeping. The course will be run in the GRETB (VEC) building in Oughterard. It start on Tuesday, 21st Feb, at 7:30pm. for more info, or bookings, please contact Ken Figgis, our education officer. Email kfiggis@hotmail.com or phone 095 41092

Bee Research Project!

FIBKA are co operating with CIT to run a beehealth project to improve the genetic diversity of Irish honey bee stocks, funding needed either corporate or individual. Click here for more info!

Kilkenny Beginners Course!

It will run for the 4 Wednesday nights (15 Feb, 22 Feb, 1st March, 8 March) from 7.30 to 9.00 covering topics such as
Beekeeping Equipment, The occupants of the hive, The beekeeping year, honey harvesting and planting for bees and pollinators.

This will be followed a number of practical sessions during the rest of the year at our own Apiary and at other Apiaries where you will get first hand experience with colonies of Bees

Contact Jer Keohane jkeohane@iece.ie for further details